Part:BBa_K2952015:Design

Translational unit for FDH_h overexpression

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BamHI site found at 1299
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal NgoMIV site found at 133
  Illegal NgoMIV site found at 526
  
• 1000
  COMPATIBLE WITH RFC[1000]

Design Notes

Codon optimisation for more efficient expression in E. coli. His-tagged for later purification using immobilised metal affinity chromatography column. Selenocysteine was replaced with cysteine to allow expression in E. coli.

Source

Genomic sequence from Clostridium carboxidivorans, which was codon-optimised for expression in E. coli.

References

Alissandratos, A., et al. (2013). Clostridium carboxidivorans Strain P7T Recombinant Formate Dehydrogenase Catalyzes Reduction of CO2 to Formate. Applied and Environmental Microbiology 79(2) 741-744.

Yoshida A., et al. (2005). Enhanced hydrogen production from formic acid by formate hydrogen lyase‐overexpressing Escherichia coli strains. Appl Environ Microbiol. 2005;71:6762–6768.